Cytological and molecular screening of Chlamydia trachomatis in infertile women attending a maternity teaching hospital in Gezira State, Sudan: a cross-sectional study

Background: Chlamydia trachomatis (CT) is a sexually transmitted pathogen that threatens reproductive health worldwide. This study aims to screen CT urogenital infection using cytology and molecular methods in women suffering infertility. Methods: In total, 415 infertility, attending Wad Madani Maternity Hospital were included this then classified into two groups: primary infertile secondary women. Both urine (n= 415) vaginal swab samples 130) collected tested Giemsa stain Polymerase Chain Reaction (PCR) for detection of CT. Results: was detected 33.7% (140/415) 73.1% (95/130) stain, compared with 44.6% (185/415) 84.6% (110/130) PCR, respectively. In the group 265), chlamydia 35.8% (95/265) 75% (60/80) by 50.9% (135/265) PCR. 150), 30% (45/150) 70% (35/50) 33.3% (50/150) 100% (50/50) The associated risk factors age, lower abdominal pain, urethritis (p< 0.05). sensitivity, specificity, positive predictive value, negative value detecting PCR 86.4%, 100%, 83.6%, respectively. Conclusions: can be used as screening test places where difficult performed.